# Chemical Defenses-1: Flores et al. 2012 # Reactive oxygen species are linked to the toxicity of the dinoflagellate Alexandrium spp. to protists # PI: Hans G. Dam # Version date: 2021-06-15 ========================= Alexandrium Densitiy Protist Filtrate Separation Mitigation Survived_protist Survival Figure ------------------------- High_PST 1000 Tiarina nd nd nd 1 6.7 Figure 1A High_PST 1000 Tiarina nd nd nd 4 26.7 Figure 1A High_PST 1000 Tiarina nd nd nd 4 26.7 Figure 1A High_PST 500 Tiarina nd nd nd 8 53.3 Figure 1A High_PST 500 Tiarina nd nd nd 6 40 Figure 1A High_PST 500 Tiarina nd nd nd 9 60 Figure 1A High_PST 250 Tiarina nd nd nd 11 73.3 Figure 1A High_PST 250 Tiarina nd nd nd 9 60 Figure 1A High_PST 250 Tiarina nd nd nd 11 73.3 Figure 1A High_PST 125 Tiarina nd nd nd 13 86.7 Figure 1A High_PST 125 Tiarina nd nd nd 11 73.3 Figure 1A High_PST 125 Tiarina nd nd nd 13 86.7 Figure 1A High_PST 63 Tiarina nd nd nd 14 93.3 Figure 1A High_PST 63 Tiarina nd nd nd 14 93.3 Figure 1A High_PST 63 Tiarina nd nd nd 13 86.7 Figure 1A Low_PST 1000 Tiarina nd nd nd 0 0 Figure 1A Low_PST 1000 Tiarina nd nd nd 0 0 Figure 1A Low_PST 1000 Tiarina nd nd nd 1 6.7 Figure 1A Low_PST 500 Tiarina nd nd nd 1 6.7 Figure 1A Low_PST 500 Tiarina nd nd nd 1 6.7 Figure 1A Low_PST 500 Tiarina nd nd nd 3 20 Figure 1A Low_PST 250 Tiarina nd nd nd 9 60 Figure 1A Low_PST 250 Tiarina nd nd nd 10 66.7 Figure 1A Low_PST 250 Tiarina nd nd nd 11 73.3 Figure 1A Low_PST 125 Tiarina nd nd nd 16 106.7 Figure 1A Low_PST 125 Tiarina nd nd nd 13 86.7 Figure 1A Low_PST 125 Tiarina nd nd nd 12 80 Figure 1A Low_PST 63 Tiarina nd nd nd 14 93.3 Figure 1A Low_PST 63 Tiarina nd nd nd 13 86.7 Figure 1A Low_PST 63 Tiarina nd nd nd 13 86.7 Figure 1A No_PST 1000 Tiarina nd nd nd 6 40 Figure 1A No_PST 1000 Tiarina nd nd nd 2 13.3 Figure 1A No_PST 1000 Tiarina nd nd nd 4 26.7 Figure 1A No_PST 500 Tiarina nd nd nd 12 80 Figure 1A No_PST 500 Tiarina nd nd nd 11 73.3 Figure 1A No_PST 500 Tiarina nd nd nd 12 80 Figure 1A No_PST 250 Tiarina nd nd nd 15 100 Figure 1A No_PST 250 Tiarina nd nd nd 13 86.7 Figure 1A No_PST 250 Tiarina nd nd nd 14 93.3 Figure 1A No_PST 125 Tiarina nd nd nd 13 86.7 Figure 1A No_PST 125 Tiarina nd nd nd 15 100 Figure 1A No_PST 125 Tiarina nd nd nd 12 80 Figure 1A No_PST 63 Tiarina nd nd nd 13 86.7 Figure 1A No_PST 63 Tiarina nd nd nd 14 93.3 Figure 1A No_PST 63 Tiarina nd nd nd 13 86.7 Figure 1A Lingulodinium 1000 Tiarina nd nd nd 16 106.7 Figure 1A Lingulodinium 1000 Tiarina nd nd nd 14 93.3 Figure 1A Lingulodinium 1000 Tiarina nd nd nd 16 106.7 Figure 1A Lingulodinium 500 Tiarina nd nd nd 14 93.3 Figure 1A Lingulodinium 500 Tiarina nd nd nd 15 100 Figure 1A Lingulodinium 500 Tiarina nd nd nd 16 106.7 Figure 1A Lingulodinium 250 Tiarina nd nd nd 13 86.7 Figure 1A Lingulodinium 250 Tiarina nd nd nd 16 106.7 Figure 1A Lingulodinium 250 Tiarina nd nd nd 14 93.3 Figure 1A Lingulodinium 125 Tiarina nd nd nd 15 100 Figure 1A Lingulodinium 125 Tiarina nd nd nd 14 93.3 Figure 1A Lingulodinium 125 Tiarina nd nd nd 16 106.7 Figure 1A Lingulodinium 63 Tiarina nd nd nd 13 86.7 Figure 1A Lingulodinium 63 Tiarina nd nd nd 15 100 Figure 1A Lingulodinium 63 Tiarina nd nd nd 13 86.7 Figure 1A FSW_Control 0 Tiarina nd nd nd 15 100 Figure 1A FSW_Control 0 Tiarina nd nd nd 14 93.3 Figure 1A FSW_Control 0 Tiarina nd nd nd 15 100 Figure 1A High_PST 1000 Polykrikos nd nd nd 4 26.7 Figure 1B High_PST 1000 Polykrikos nd nd nd 2 13.3 Figure 1B High_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B High_PST 500 Polykrikos nd nd nd 7 46.7 Figure 1B High_PST 500 Polykrikos nd nd nd 4 26.7 Figure 1B High_PST 500 Polykrikos nd nd nd 13 86.7 Figure 1B High_PST 250 Polykrikos nd nd nd 17 113.3 Figure 1B High_PST 250 Polykrikos nd nd nd 11 73.3 Figure 1B High_PST 250 Polykrikos nd nd nd 24 160 Figure 1B High_PST 125 Polykrikos nd nd nd 23 153.3 Figure 1B High_PST 125 Polykrikos nd nd nd 19 126.7 Figure 1B High_PST 125 Polykrikos nd nd nd 27 180 Figure 1B High_PST 63 Polykrikos nd nd nd 28 186.7 Figure 1B High_PST 63 Polykrikos nd nd nd 27 180 Figure 1B High_PST 63 Polykrikos nd nd nd 25 166.7 Figure 1B Low_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 500 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 500 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 500 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 250 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 250 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 250 Polykrikos nd nd nd 0 0 Figure 1B Low_PST 125 Polykrikos nd nd nd 9 60 Figure 1B Low_PST 125 Polykrikos nd nd nd 6 40 Figure 1B Low_PST 125 Polykrikos nd nd nd 8 53.3 Figure 1B Low_PST 63 Polykrikos nd nd nd 9 60 Figure 1B Low_PST 63 Polykrikos nd nd nd 7 46.7 Figure 1B Low_PST 63 Polykrikos nd nd nd 9 60 Figure 1B No_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B No_PST 1000 Polykrikos nd nd nd 2 13.3 Figure 1B No_PST 1000 Polykrikos nd nd nd 0 0 Figure 1B No_PST 500 Polykrikos nd nd nd 6 40 Figure 1B No_PST 500 Polykrikos nd nd nd 2 13.3 Figure 1B No_PST 500 Polykrikos nd nd nd 4 26.7 Figure 1B No_PST 250 Polykrikos nd nd nd 19 126.7 Figure 1B No_PST 250 Polykrikos nd nd nd 16 106.7 Figure 1B No_PST 250 Polykrikos nd nd nd 18 120 Figure 1B No_PST 125 Polykrikos nd nd nd 19 126.7 Figure 1B No_PST 125 Polykrikos nd nd nd 20 133.3 Figure 1B No_PST 125 Polykrikos nd nd nd 21 140 Figure 1B No_PST 63 Polykrikos nd nd nd 23 153.3 Figure 1B No_PST 63 Polykrikos nd nd nd 16 106.7 Figure 1B No_PST 63 Polykrikos nd nd nd 16 106.7 Figure 1B Lingulodinium 1000 Polykrikos nd nd nd 24 160 Figure 1B Lingulodinium 1000 Polykrikos nd nd nd 27 180 Figure 1B Lingulodinium 1000 Polykrikos nd nd nd 26 173.3 Figure 1B Lingulodinium 500 Polykrikos nd nd nd 26 173.3 Figure 1B Lingulodinium 500 Polykrikos nd nd nd 20 133.3 Figure 1B Lingulodinium 500 Polykrikos nd nd nd 26 173.3 Figure 1B Lingulodinium 250 Polykrikos nd nd nd 26 173.3 Figure 1B Lingulodinium 250 Polykrikos nd nd nd 27 180 Figure 1B Lingulodinium 250 Polykrikos nd nd nd 24 160 Figure 1B Lingulodinium 125 Polykrikos nd nd nd 29 193.3 Figure 1B Lingulodinium 125 Polykrikos nd nd nd 28 186.7 Figure 1B Lingulodinium 125 Polykrikos nd nd nd 31 206.7 Figure 1B Lingulodinium 63 Polykrikos nd nd nd 18 120 Figure 1B Lingulodinium 63 Polykrikos nd nd nd 20 133.3 Figure 1B Lingulodinium 63 Polykrikos nd nd nd 22 146.7 Figure 1B FSW_Control 0 Polykrikos nd nd nd 21 140 Figure 1B FSW_Control 0 Polykrikos nd nd nd 16 106.7 Figure 1B FSW_Control 0 Polykrikos nd nd nd 22 146.7 Figure 1B High_PST 1000 Tiarina 0.2um nd nd 14 93.3 Figure 2 High_PST 1000 Tiarina 0.2um nd nd 12 80 Figure 2 High_PST 1000 Tiarina 0.2um nd nd 13 86.7 Figure 2 High_PST 1000 Tiarina 5um nd nd 13 86.7 Figure 2 High_PST 1000 Tiarina 5um nd nd 16 106.7 Figure 2 High_PST 1000 Tiarina 5um nd nd 14 93.3 Figure 2 High_PST 1000 Tiarina extract nd nd 13 86.7 Figure 2 High_PST 1000 Tiarina extract nd nd 14 93.3 Figure 2 High_PST 1000 Tiarina extract nd nd 15 100 Figure 2 Low_PST 1000 Tiarina 0.2um nd nd 14 93.3 Figure 2 Low_PST 1000 Tiarina 0.2um nd nd 16 106.7 Figure 2 Low_PST 1000 Tiarina 0.2um nd nd 14 93.3 Figure 2 Low_PST 1000 Tiarina 5um nd nd 5 33.3 Figure 2 Low_PST 1000 Tiarina 5um nd nd 6 40 Figure 2 Low_PST 1000 Tiarina 5um nd nd 4 26.7 Figure 2 Low_PST 1000 Tiarina extract nd nd 14 93.3 Figure 2 Low_PST 1000 Tiarina extract nd nd 15 100 Figure 2 Low_PST 1000 Tiarina extract nd nd 14 93.3 Figure 2 No_PST 1000 Tiarina 0.2um nd nd 12 80 Figure 2 No_PST 1000 Tiarina 0.2um nd nd 15 100 Figure 2 No_PST 1000 Tiarina 0.2um nd nd 16 106.7 Figure 2 No_PST 1000 Tiarina 5um nd nd 11 73.3 Figure 2 No_PST 1000 Tiarina 5um nd nd 15 100 Figure 2 No_PST 1000 Tiarina 5um nd nd 13 86.7 Figure 2 No_PST 1000 Tiarina extract nd nd 12 80 Figure 2 No_PST 1000 Tiarina extract nd nd 16 106.7 Figure 2 No_PST 1000 Tiarina extract nd nd 13 86.7 Figure 2 FSW_Control 0 Tiarina 0.2um nd nd 15 100 Figure 2 FSW_Control 0 Tiarina 0.2um nd nd 16 106.7 Figure 2 FSW_Control 0 Tiarina 0.2um nd nd 16 106.7 Figure 2 FSW_Control 0 Tiarina 5um nd nd 14 93.3 Figure 2 FSW_Control 0 Tiarina 5um nd nd 12 80 Figure 2 FSW_Control 0 Tiarina 5um nd nd 13 86.7 Figure 2 High_PST 1000 Tiarina nd mesh nd 2 13.3 Figure 3 High_PST 1000 Tiarina nd mesh nd 2 13.3 Figure 3 High_PST 1000 Tiarina nd mesh nd 3 20 Figure 3 Low_PST 1000 Tiarina nd mesh nd 0 0 Figure 3 Low_PST 1000 Tiarina nd mesh nd 1 6.7 Figure 3 Low_PST 1000 Tiarina nd mesh nd 0 0 Figure 3 No_PST 1000 Tiarina nd mesh nd 2 13.3 Figure 3 No_PST 1000 Tiarina nd mesh nd 1 6.7 Figure 3 No_PST 1000 Tiarina nd mesh nd 4 26.7 Figure 3 High_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 High_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 High_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 Low_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 Low_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 Low_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 No_PST 1000 Tiarina nd no_mesh nd 1 6.7 Figure 3 No_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 No_PST 1000 Tiarina nd no_mesh nd 0 0 Figure 3 FSW_Control 0 Tiarina nd mesh nd 12 80 Figure 3 FSW_Control 0 Tiarina nd mesh nd 13 86.7 Figure 3 FSW_Control 0 Tiarina nd mesh nd 13 86.7 Figure 3 High_PST 1000 Tiarina nd nd No_addition 0 0 Figure 4A High_PST 1000 Tiarina nd nd No_addition 1 6.7 Figure 4A High_PST 1000 Tiarina nd nd No_addition 0 0 Figure 4A Low_PST 1000 Tiarina nd nd No_addition 0 0 Figure 4A Low_PST 1000 Tiarina nd nd No_addition 0 0 Figure 4A Low_PST 1000 Tiarina nd nd No_addition 0 0 Figure 4A No_PST 1000 Tiarina nd nd No_addition 1 6.7 Figure 4A No_PST 1000 Tiarina nd nd No_addition 2 13.3 Figure 4A No_PST 1000 Tiarina nd nd No_addition 2 13.3 Figure 4A FSW_Control 0 Tiarina nd nd No_addition 14 93.3 Figure 4A FSW_Control 0 Tiarina nd nd No_addition 13 86.7 Figure 4A FSW_Control 0 Tiarina nd nd No_addition 16 106.7 Figure 4A High_PST 1000 Tiarina nd nd Peroxidase 11 73.3 Figure 4A High_PST 1000 Tiarina nd nd Peroxidase 11 73.3 Figure 4A High_PST 1000 Tiarina nd nd Peroxidase 10 66.7 Figure 4A Low_PST 1000 Tiarina nd nd Peroxidase 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Peroxidase 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Peroxidase 0 0 Figure 4A No_PST 1000 Tiarina nd nd Peroxidase 5 33.3 Figure 4A No_PST 1000 Tiarina nd nd Peroxidase 7 46.7 Figure 4A No_PST 1000 Tiarina nd nd Peroxidase 7 46.7 Figure 4A FSW_Control 1000 Tiarina nd nd Peroxidase 14 93.3 Figure 4A FSW_Control 1000 Tiarina nd nd Peroxidase 15 100 Figure 4A FSW_Control 1000 Tiarina nd nd Peroxidase 14 93.3 Figure 4A High_PST 1000 Tiarina nd nd Catalase 1 6.7 Figure 4A High_PST 1000 Tiarina nd nd Catalase 0 0 Figure 4A High_PST 1000 Tiarina nd nd Catalase 1 6.7 Figure 4A Low_PST 1000 Tiarina nd nd Catalase 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Catalase 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Catalase 0 0 Figure 4A No_PST 1000 Tiarina nd nd Catalase 4 26.7 Figure 4A No_PST 1000 Tiarina nd nd Catalase 1 6.7 Figure 4A No_PST 1000 Tiarina nd nd Catalase 6 40 Figure 4A FSW_Control 0 Tiarina nd nd Catalase 15 100 Figure 4A FSW_Control 0 Tiarina nd nd Catalase 17 113.3 Figure 4A FSW_Control 0 Tiarina nd nd Catalase 14 93.3 Figure 4A High_PST 1000 Tiarina nd nd SOD 4 26.7 Figure 4A High_PST 1000 Tiarina nd nd SOD 4 26.7 Figure 4A High_PST 1000 Tiarina nd nd SOD 6 40 Figure 4A Low_PST 1000 Tiarina nd nd SOD 0 0 Figure 4A Low_PST 1000 Tiarina nd nd SOD 0 0 Figure 4A Low_PST 1000 Tiarina nd nd SOD 0 0 Figure 4A No_PST 1000 Tiarina nd nd SOD 10 66.7 Figure 4A No_PST 1000 Tiarina nd nd SOD 5 33.3 Figure 4A No_PST 1000 Tiarina nd nd SOD 7 46.7 Figure 4A FSW_Control 0 Tiarina nd nd SOD 16 106.7 Figure 4A FSW_Control 0 Tiarina nd nd SOD 16 106.7 Figure 4A FSW_Control 0 Tiarina nd nd SOD 20 133.3 Figure 4A High_PST 1000 Tiarina nd nd Trypsin 1 6.7 Figure 4A High_PST 1000 Tiarina nd nd Trypsin 0 0 Figure 4A High_PST 1000 Tiarina nd nd Trypsin 1 6.7 Figure 4A Low_PST 1000 Tiarina nd nd Trypsin 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Trypsin 0 0 Figure 4A Low_PST 1000 Tiarina nd nd Trypsin 0 0 Figure 4A No_PST 1000 Tiarina nd nd Trypsin 10 66.7 Figure 4A No_PST 1000 Tiarina nd nd Trypsin 8 53.3 Figure 4A No_PST 1000 Tiarina nd nd Trypsin 7 46.7 Figure 4A FSW_Control 0 Tiarina nd nd Trypsin 14 93.3 Figure 4A FSW_Control 0 Tiarina nd nd Trypsin 15 100 Figure 4A FSW_Control 0 Tiarina nd nd Trypsin 15 100 Figure 4A High_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B High_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B High_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd No_addition 0 0 Figure 4B No_PST 1000 Polykrikos nd nd No_addition 4 26.7 Figure 4B No_PST 1000 Polykrikos nd nd No_addition 3 20 Figure 4B No_PST 1000 Polykrikos nd nd No_addition 4 26.7 Figure 4B FSW_Control 0 Polykrikos nd nd No_addition 13 86.7 Figure 4B FSW_Control 0 Polykrikos nd nd No_addition 12 80 Figure 4B FSW_Control 0 Polykrikos nd nd No_addition 14 93.3 Figure 4B High_PST 1000 Polykrikos nd nd Peroxidase 17 113.3 Figure 4B High_PST 1000 Polykrikos nd nd Peroxidase 12 80 Figure 4B High_PST 1000 Polykrikos nd nd Peroxidase 12 80 Figure 4B Low_PST 1000 Polykrikos nd nd Peroxidase 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Peroxidase 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Peroxidase 0 0 Figure 4B No_PST 1000 Polykrikos nd nd Peroxidase 10 66.7 Figure 4B No_PST 1000 Polykrikos nd nd Peroxidase 8 53.3 Figure 4B No_PST 1000 Polykrikos nd nd Peroxidase 9 60 Figure 4B High_PST 1000 Polykrikos nd nd Catalase 1 6.7 Figure 4B High_PST 1000 Polykrikos nd nd Catalase 0 0 Figure 4B High_PST 1000 Polykrikos nd nd Catalase 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Catalase 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Catalase 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Catalase 0 0 Figure 4B No_PST 1000 Polykrikos nd nd Catalase 1 6.7 Figure 4B No_PST 1000 Polykrikos nd nd Catalase 1 6.7 Figure 4B No_PST 1000 Polykrikos nd nd Catalase 1 6.7 Figure 4B High_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B High_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B High_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd SOD 0 0 Figure 4B No_PST 1000 Polykrikos nd nd SOD 5 33.3 Figure 4B No_PST 1000 Polykrikos nd nd SOD 6 40 Figure 4B No_PST 1000 Polykrikos nd nd SOD 7 46.7 Figure 4B High_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B High_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B High_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B Low_PST 1000 Polykrikos nd nd Trypsin 0 0 Figure 4B No_PST 1000 Polykrikos nd nd Trypsin 6 40 Figure 4B No_PST 1000 Polykrikos nd nd Trypsin 5 33.3 Figure 4B No_PST 1000 Polykrikos nd nd Trypsin 7 46.7 Figure 4B